Sensitive and modular amplicon sequencing of Plasmodium falciparum diversity and resistance for research and public health

dc.contributor.authorAranda-Diaz, Andres
dc.contributor.authorVickers, Eric Neubauer
dc.contributor.authorMurie, Kathryn
dc.contributor.authorPalmer, Brian
dc.contributor.authorHathaway, Nicholas
dc.contributor.authorGerlovina, Inna
dc.contributor.authorBoene, Simone
dc.contributor.authorGarcia-Ulloa, Manuel
dc.contributor.authorCistero, Pau
dc.contributor.authorKatairo, Thomas
dc.contributor.authorSemakuba, Francis Ddumba
dc.contributor.authorNsengimaana, Bienvenu
dc.contributor.authorGwarinda, Hazel
dc.contributor.authorGarcia-Fernandez, Carla
dc.contributor.authorLouie, William
dc.contributor.authorEsayas, Endashaw
dc.contributor.authorDa Silva, Clemente
dc.contributor.authorDatta, Debayan
dc.contributor.authorKiyaga, Shahiid
dc.contributor.authorWiringilimaana, Innocent
dc.contributor.authorFeleke, Sindew Mekasha
dc.contributor.authorBennett, Adam
dc.contributor.authorSmith, Jennifer L.
dc.contributor.authorGadisa, Endalamaw
dc.contributor.authorParr, Jonathan B.
dc.contributor.authorConrad, Melissa D.
dc.contributor.authorRaman, Jaishree
dc.contributor.authorTukwasibwe, Stephen
dc.contributor.authorSsewanyana, Isaac
dc.contributor.authorRovira-Vallbona, Eduard
dc.contributor.authorTato, Cristina M.
dc.contributor.authorBriggs, Jessica
dc.contributor.authorMayor, Alfredo
dc.contributor.authorGreenhouse, Bryan
dc.date.accessioned2025-09-12T08:08:21Z
dc.date.available2025-09-12T08:08:21Z
dc.date.issued2025-03
dc.descriptionDATA AVAILABILITY: All data are available in the Sequencing Read Archive, accession code PRJNA1180199. Code is available in GitHub (https://github.com/EPPIcenter/mad4hatter and https://github.com/andres-ad/madh_utilities).
dc.description.abstractPlease read abstract in the article.
dc.description.departmentUP Centre for Sustainable Malaria Control (UP CSMC)
dc.description.librarianhj2025
dc.description.sdgSDG-03: Good health and well-being
dc.description.sponsorshipGrants from the Bill & Melinda Gates Foundation. This research is also part of the ISGlobal’s Program on the Molecular Mechanisms of Malaria which is partially supported by the Fundación Ramón Areces. Support the Generalitat de Catalunya through the CERCA Program, from the Departament d’Universitats i Recerca de la Generalitat de Catalunya; the Ministerio de Ciencia e Innovación; the Government of Mozambique; the Spanish Agency for International Development (AECID); the Chan Zuckerberg Biohub Collaborative Postdoctoral fellowship; and NIH-NIAID. The parent study from which the 2017–2018 Ethiopia samples were derived was funded by the Global Fund to Fight AIDS, Tuberculosis, and Malaria through the Ministry of Health - Ethiopia (EPHI5405) and by the Bill & Melinda Gates Foundation through the World Health Organization.
dc.description.urihttps://www.nature.com/srep
dc.identifier.citationAranda-Díaz, A., Neubauer Vickers, E., Murie, K. et al. Sensitive and modular amplicon sequencing of Plasmodium falciparum diversity and resistance for research and public health. Scientific Reports 15, 10737 (2025). https://doi.org/10.1038/s41598-025-94716-5.
dc.identifier.issn2045-2322 (online)
dc.identifier.other10.1038/s41598-025-94716-5
dc.identifier.urihttp://hdl.handle.net/2263/104307
dc.language.isoen
dc.publisherNature Portfolio
dc.rights© The Author(s) 2025. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.
dc.subjectMalaria
dc.subjectPlasmodium falciparum
dc.subjectTargeted amplicon sequencing
dc.subjectMicrohaplotype
dc.subjectAntimalarial resistance
dc.subjecthrp2 deletion
dc.subjectCsp
dc.subjectIdentity-by-descent (IBD)
dc.subjectComplexity of infection
dc.subjectWhole-genome sequencing (WGS)
dc.titleSensitive and modular amplicon sequencing of Plasmodium falciparum diversity and resistance for research and public health
dc.typeArticle

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